The aim of this experiment is to investigate the effects of acidic protease on the egg laying perform-ance,egg quality,antioxidant capacity,and immune function of laying hens.A total of 600 Beijing Red laying hens,aged 900 days and forced to molt for 30 days,were randomly divided into 4 groups,with 6 replicates in each group and 25 hens in each replicate.The control group was fed with basic feed,while experimental groups I,II,and III were supplemented with 250 g/t,500 g/t,and 750 g/t acid protease in the feed,respectively.The pre tri-al period and the main trial period are 1 week and 6 weeks,respectively.The results showed that compared with the control group,the egg production rate of experimental group II significantly increased(P<0.05),while the feed to egg ratio and egg breakage rate significantly decreased(P<0.05);The eggshell thickness,Hartmann u-nits,and eggshell strength of experimental groups II and III were significantly increased(P<0.05);The serum levels of IgM,IgG,and GSH Px in three experimental groups of laying hens were significantly increased(P<0.05).In summary,adding an appropriate amount of acidic protease to the feed can improve the egg laying per-formance and quality of laying hens,as well as enhance their immune performance and antioxidant capacity.The recommended dosage is 500 g/t.
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