Effects of Simiao Powder and Its Disassembled Prescription on Serum IL-6 and MMP-3 in Rats with Rheumatoid Arthritis
Objective:To observe the effects of the classic RA animal model serum IL-6 and MMP-3 with the ＂Simiao Powder＂and its disassembled formulas,in order to understand the therapeutic efficacy of＂Simiao Powder＂for RA and its composition characteristics.Methods:100 female SD rats were randomly divided into 10 groups,including a control group,a model group,a western medicine group(methotrexate,MTX),a＂Simiao Decoction＂group,and six disassembled formula groups(Phellodendron+Achyranthes,Atractylodes+Achyranthes,Coix Seed+Achyranthes,Atractylodes+Coix Seed,Phellodendron+Coix Seed,and Phellodendron+Atractylodes).Each group had 10 rats.Except for the blank group,the remaining rats were induced with arthritis models by injecting 0.1 ml of Freund's complete adjuvant on the right foot.Starting from the first day after modeling,drug intervention began in each group.The normal control group and model group were given equal volumes of double-distilled water by gavage.All rats were administered according to a dosage of 10 ml/kg once daily for 14 consecutive days.After the gavage treatment was completed,the levels of IL-6 and MMP-3 in the serum of each group were measured using the ELISA method.Results:The levels of IL-6 and MMP-3 in the serum of rats in the ＂Simiao Powder＂group and its disassembled formulas decreased to varying degrees(P＜0.05).According to the comprehensive index results,the ＂Simiao Powder＂group had the best effect,followed by the Phellodendron+Achyranthes group,Phellodendron+Atractylodes group,At-ractylodes+Achyranthes group,Phellodendron+Coix Seed group,Coix Seed+Achyranthes group,and At-ractylodes+Coix Seed group.Conclusion:The combined application of the ＂Simiao Powder＂has exhibited optimal efficacy in reducing serum inflammatory factors in adjuvant-induced RA rats.The strongest effect is observed in the Phellodendron+Achyranthes group.The ＂Simiao Powder＂group has a notable characteristic of concise and specialized composition.Among the pairwise combinations,the Phellodendron+Achyranthes group has the strongest effect in reducing serum IL-6 and MMP-3 levels in rats.This provides new insights into the dominant and supporting components of＂Simiao Powder＂,but further clinical studies are needed to confirm these findings.
NETL
NSTL
万方数据
维普
