摘要
目的:观察肉苁蓉骨靶向纳米粒促进大鼠骨缺损的修复作用.方法:选取清洁级雄性SD大鼠40 只,采用随机数字表法随机分成 4 组,分别设立正常对照组、四环素纳米粒(TC-PLGANP)组、肉苁蓉纳米粒(CH/PL-GANP)组、肉苁蓉骨靶向纳米粒(CH/TC-PLGANP)组,每组 10 只.除正常对照组外,每组按照相应方法建立大鼠股骨缺损模型.药物干预后,应用蛋白印迹法和免疫组化法检测骨组织中的FGF-2、VEGF、BMP-2 表达量.结果:与正常对照组比较,TC-PLGANP组、CH/PLGANP组、CH/TC-PLGANP组大鼠骨组织FGF-2、VEGF、BMP-2 的蛋白相对表达量、阳性颗粒相对表达量明显升高(P＜0.05);与TC-PLGANP组比较,CH/PLGANP组、CH/TC-PL-GANP组大鼠骨组织FGF-2、VEGF、BMP-2 的蛋白相对表达量、阳性颗粒相对表达量明显升高(P＜0.05);与CH/PLGANP组比较,CH/TC-PLGANP组大鼠骨组织FGF-2、VEGF、BMP-2 的蛋白相对表达量、阳性颗粒相对表达量明显升高(P＜0.05).结论:肉苁蓉骨靶向制剂相较肉苁蓉普通制剂而言,骨组织中促进骨修复因子表达量更高,有利于骨缺损的修复.
Abstract
Objective:To observe the effect of Cistanche deserticola bone targeted nanoparticles on pro-moting the repair of bone defects in rats.Methods:Forty clean male SD rats were randomly divided into four groups using a random number table method,namely a normal control group,tetracycline nanoparticles(TC-PLGANP)group,Cistanche deserticola nanoparticles(CH/PLGANP)group,and Cistanche deserticola bone targeted nanoparticles(CH/TC-PLGANP)group,with 10 rats in each group.Except for the normal control group,each group was established using a corresponding method to establish a rat femoral defect model.After drug intervention,western blot and immunohistochemical methods were used to detect the expression levels of FGF-2,VEGF,and BMP-2 in bone tissues.Results:Compared with the normal control group,the relative expression of FGF-2,VEGF,and BMP-2 proteins and the relative expression of positive particles in bone tis-sues of rats in the TC-PLGANP group,CH/PLGANP group,and CH/TC-PLGANP group significantly in-creased(P＜0.05).Compared with the TC-PLGANP group,the relative expression of FGF-2,VEGF,and BMP-2 proteins and the relative expression of positive particles in bone tissues of rats in the CH/PLGANP group and CH/TC-PLGANP group significantly increased(P＜0.05).Compared with the CH/PLGANP group,the relative expression of FGF-2,VEGF,and BMP-2 proteins and the relative expression of positive particles in bone tissues of rats in the CH/TC-PLGANP group significantly increased(P＜0.05).Conclu-sion:Compared with ordinary preparations of Cistanche deserticola,bone targeted preparations of Cistanche de-serticola have higher expression levels of bone repair factors in bone tissues,which is beneficial to the repair of bone defects.
基金项目
国家重点研发计划"中医药现代化"重点专项(2019)(2019YFC1712500)
黔南民族医学高等专科学校科研项目(QNYZ201815)
黔南民族医学高等专科学校科研项目(QNYZ202210)
黔南民族医学高等专科学校大学生创新项目(QNYZ202204)
NETL
NSTL
维普
万方数据