Experimental Study on Cistanche deserticola Bone Targeting Nanoparticles Promoting Bone Defect Repair in Rats
Objective:To observe the effect of Cistanche deserticola bone targeted nanoparticles on pro-moting the repair of bone defects in rats.Methods:Forty clean male SD rats were randomly divided into four groups using a random number table method,namely a normal control group,tetracycline nanoparticles(TC-PLGANP)group,Cistanche deserticola nanoparticles(CH/PLGANP)group,and Cistanche deserticola bone targeted nanoparticles(CH/TC-PLGANP)group,with 10 rats in each group.Except for the normal control group,each group was established using a corresponding method to establish a rat femoral defect model.After drug intervention,western blot and immunohistochemical methods were used to detect the expression levels of FGF-2,VEGF,and BMP-2 in bone tissues.Results:Compared with the normal control group,the relative expression of FGF-2,VEGF,and BMP-2 proteins and the relative expression of positive particles in bone tis-sues of rats in the TC-PLGANP group,CH/PLGANP group,and CH/TC-PLGANP group significantly in-creased(P＜0.05).Compared with the TC-PLGANP group,the relative expression of FGF-2,VEGF,and BMP-2 proteins and the relative expression of positive particles in bone tissues of rats in the CH/PLGANP group and CH/TC-PLGANP group significantly increased(P＜0.05).Compared with the CH/PLGANP group,the relative expression of FGF-2,VEGF,and BMP-2 proteins and the relative expression of positive particles in bone tissues of rats in the CH/TC-PLGANP group significantly increased(P＜0.05).Conclu-sion:Compared with ordinary preparations of Cistanche deserticola,bone targeted preparations of Cistanche de-serticola have higher expression levels of bone repair factors in bone tissues,which is beneficial to the repair of bone defects.
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