Application Value of Chemiluminescence Assay and Hepatitis B Virus DNA Fluorescence Quantification in the Detection of Hepatitis B
Objective:To clarify the value of chemiluminescence immunoassay(CLIA)and hepatitis B virus(HBV)DNA fluorescence quantification in the detection of hepatitis B.Methods:A total of 285 patients with hepatitis B and 280 patients with liver cirrhosis were selected as subjects.Samples from both groups were analyzed using CLIA and HBV DNA fluorescence quantification,with relevant testing data compiled and statisti-cally analyzed.Results:Levels of HBsAg,HBsAb,HBeAg,HBeAb,and HBcAb were significantly lower in the hepatitis B group compared to the liver cirrhosis group(P<0.05),whereas HBV-DNA levels were sign-ificantly higher.As HBV-DNA levels increased,various liver function indicators for hepatitis B patients also showed corresponding elevations to varying degrees(P<0.05).Pearson correlation analysis revealed negative correlations between HBV-DNA and HBsAg,HBsAb,HBeAg,HBeAb,and HBcAb(t=-0.876,-0.956,-0.974,-0.922,-0.981,respectively).Conclusion:Both CLIA and HBV DNA fluorescence quantification techniques demonstrate value in detecting hepatitis B,showing a negative correlation between them.These methods can provide reference information for the diagnosis and prognosis assessment of hepatitis B.
Hepatitis BViral DNAFluorescence QuantificationChemiluminescence ImmunoassayLiver Function
万方数据
维普
