Comparison of polyphenol compounds in Gastrodia from different producing areas based on UHPLC
The aim of this study was to analyze the content of above polyphenols in Gastrodia in different ecological areas by ultra-high pressure liquid chromatography,and to compare the differences of polyphenols in Gastrodia in different areas by analyzing the polyphenol fingerprints,and to evaluate the quality of Gastrodia.The super high-performance liquid chromatography of polyphenols Gastrodia elBlume was determined by ultra-violet detection and gradient elution,and the peak area was stable within 24 hours,the relative standard deviation was 1.03％(n=5).The concentrations of gastrodin,vanillin,p-hydroxybenzoic acid and syringaldehyde in Gastrodia elata from Shanxi,Jiangsu,Yunnan,Guizhou,Sichuan,Guangxi and Jilin were 0.341,0.018,0.013,0.026mg·g-1,0.183,0.019,0,0.026 mg·g-1,0.207,0.009,0,0mg·g-1,0.238,0.026,0,0.025 mg·g-1,0.190,0.018,0,0.013mg·g-1,0.365,0.028,0.026,0.026 mg·g-1,0.357,0.028,0.013,0.027mg·g-1.Coumaric acid,ferulic acid and caffeic acid were not detected in all samples.The sequence of total polyphenols in Gastrodia elata from high to low is Guangxi,Jilin,Shanxi,Guizhou,Jiangsu,Sichuan,and Yunnan.The content of each polyphenol was in the order of gastrodin,vanillin,syringaldehyde and p-hydroxybenzoic acid,which provided some theoretical basis and reference value for the development of Gastrodia polyphenols and the quality identification of Gastrodia.
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