Bioinformatics and expression analysis of SlSUTs gene family in tomato
The gene and protein sequences of tomato SUTs gene family members were comprehensively analyzed by bioinformatics methods and the expression characteristics of those genes were analyzed.There are three members in tomato SlSUTs gene family,and the molecular weight of protein is 54.15～64.99 kD.These three genes are evenly distributed on chromosomes 4,5 and 11.There are three patterns of intron and exon substructure of their genes,with significant differences.Both tomato SlSUTs and Arabidopsis AtSUTs amino acids have 2 forms of motifs existence,and their positions are consistent.There are four secondary structures of SUTs proteins in both tomato and Arabidopsis,among which a proportion of spirals is the highest,the proportion of β-pleated sheet is the least.Those proteins have 11 or 12 transmembrane domains,and the prediction of subcellular localization indicated that all members were located on the cell membrane.The evolutionary relationship of plant SUTs genes follows the phylogenetic relationships of species and genera,while the evolutionary relationship between tomato SlSUTs genes and grapes is more advanced.The expression of SlSUTs genes in tomato varies in different tissues and fruit development stages,indicating that the expression of SlSUTs genes have certain tissue specificity.
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