两亲荧光芘衍生物与牛血清白蛋白的相互作用
Interaction of amphiphilic pyrene derivatives with bovine serum albumin
赵新宇 1陈林 1杨瑞 1赵振龙 1张宇 1贾丽华1
作者信息
- 1. 齐齐哈尔大学化学与化学工程学院,黑龙江齐齐哈尔 161006
- 折叠
摘要
为了获得水溶性芘衍生物与血清白蛋白的结合特性,研究了两亲芘衍生物(C12PDA)与牛血清白蛋白(BSA)在中性缓冲溶液中的相互作用.首先,通过吸收和荧光光谱研究了C12PDA与BSA的相互作用.结果表明,C12PDA对BSA的荧光猝灭属于静态猝灭,疏水作用是两者之间的主要作用力.进一步利用同步荧光、三维荧光、圆二色光谱及分子对接模拟计算方法,研究了C12PDA对BSA构象的影响以及结合方式.结果表明,C12PDA与BSA的多个氨酸残基存在分子间氢键等相互作用,C12PDA使BSA的α-螺旋结构含量减少.
Abstract
In order to obtain the binding properties of water-soluble pyrene derivatives with serum albumin,the interaction between a pyrene derivative(C12PDA)and bovine serum albumin(BSA)in neutral buffer solution was studied.Firstly,the interaction between C12PDA and BSA was studied by absorption and fluorescence spectra.The results show that the fluorescence of BSA is quenched by C12PDA due to static quenching,and the hydrophobic effect is the dominant force in the complex forming.The effect of C12PDA on the conformation of BSA and its binding mode were studied by synchronous fluorescence,three-dimensional fluorescence,circular dichroism and molecular docking simulation.The results showed that there were intermolecular hydrogen bond interactions between C12PDA and several amino acid residues of BSA.Moreover,the content of α-helical structure of BSA was reduced by C12PDA.
关键词
芘/荧光/光谱/牛血清白蛋白/相互作用Key words
pyrene/fluorescence/spectrum/bovine serum albumin/interaction引用本文复制引用
基金项目
黑龙江省省属高校基本科研业务费项目(145109102)
出版年
2024
NETL
NSTL
万方数据