Optimization of ISSR-PCR reaction system and genetic characteristics analysis of Ice Lavandula 2 lavender
For exploring the genetic characteristics of Ice Lavandula 2 lavender(Lavandula angustifolia),ISSR molecular marker technology was used for the analysis,the optimal ISSR-PCR amplification system optimized for Ice Lavandula 2 was as follows:in the 20 μL system,the amount of dNTP(2.5 mmol/L each)was 1.4 μL,the amount of primer(10 μmol/L)was 0.7 μL,the amount of DNA template was 20 ng,and the amount of Taq DNA polymerase was 0.75 μmol/min.Eight ISSR primers were selected for the tests of the parents and F1 generation individuals of Ice Lavandula 2,and the genetic diversity index of the parents and F1 generation individuals was calculated and analyzed according to the amplified results.The results indicated that the genetic diversity and genetic variation of Ice Lavandula 2 was low.
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