8个大豆SERK基因干旱胁迫表达分析
Expression analysis of 8 SERK genes in soybean under drought stress
关鑫宇 1林阳 1陈炯辛 1马洪宇 1张勇 2翟莹1
作者信息
- 1. 齐齐哈尔大学生命科学与农林学院,黑龙江齐齐哈尔 161006
- 2. 黑龙江省农业科学院克山分院,黑龙江齐齐哈尔 161606
- 折叠
摘要
对大豆中的8个GmSERK基因及其启动子序列进行生物信息学分析,通过实时荧光定量RT-PCR(qRT-PCR)对基因在干旱胁迫下的表达量进行检测.结果显示,8个GmSERK基因分别来自大豆基因组的5条染色体,它们编码的蛋白含有596～624个氨基酸.8个GmSERK蛋白均含有1个信号肽序列和多个亮氨酸富集重复序列(LRR).除GmSERK10蛋白不具有跨膜结构域外,其他GmSERK蛋白均含有1个跨膜结构域和1个激酶结构域.除GmSERK7外,其余7个GmSERK基因在干旱胁迫下的表达量均发生变化,表明大豆SERK基因家族中的部分基因能够响应干旱胁迫.8个GmSERK启动子中共含有5种激素响应相关元件(ABRE、CGTCA-motif、P-box、TATC-box和TCA-element)和3种胁迫响应相关元件(ARE、MBS和TC-rich repeats),但不同启动子中含有的元件种类和数量各不相同.
Abstract
In this study,eight soybean GmSERK genes and their promoter sequences were analyzed by bioinformatics,and their expression levels under drought stress were detected by real-time fluorescence quantitative RT-PCR(qRT-PCR).The results showed that eight GmSERK genes were located on five chromosomes of soybean genome,respectively,and the proteins they encoded containing 596-624 amino acids.The eight GmSERK proteins all contained one signal peptide sequence and multiple leucine-enriched repeats(LRR).Except for GmSERK10 protein,all other GmSERK proteins contain one transmembrane domain and one kinase domain.Except for GmSERK7,the expression levels of the other 7 GmSERK genes changed under drought stress,indicating that some soybean GmSERK genes could respond to drought stress.The eight GmSERK promoters contained five type of hormone response-related elements(ABRE,CGTCA-motif,P-box,TATC-box and TCA-element)and three type of stress response-related elements(ARE,MBS and TC-rich repeats).However,the type and number of elements contained in different promoters are different.
关键词
大豆/SERK/生物信息学分析/干旱胁迫/启动子Key words
soybean/SERK/bioinformatics analysis/drought stress/promoter引用本文复制引用
基金项目
齐齐哈尔大学大学生创新创业训练计划项目(x202310232033)
黑龙江省省属高等学校基本科研业务费科研项目(145109506)
出版年
2024
NETL
NSTL
万方数据