Objective The clinical research analyzes the use of antibiotics in the treatment of neonatal pneumonia. Methods Analyzing the clinical data of antibiotic treatment of 100cases’neonatal pneumonia during 2010-2012, recording time effect on neonatal pneumonia in children with specific antibiotic therapy and different therapeutic drugs. Results Cefotaxime sodium, penicillin and ampicillin are mainly used in the treatment of neonatal pneumonia, respectively accounting for 23%、20% and 18%. Conclusion Penicillin drugs have a good effect in the treatment of neonatal pneumonia,and cephalosporin antibiotics are used more widely.