A dye,(E)-4-(4-(dimethylamino)styrene)benzonitrile,was synthesized from 4-cyanobenzyl bromide by introdu-cing electron-withdrawing group cyano and electron-donating group dimethylamino into stilbene.The photophysical properties and theo-retical calculations of the dye were studied,and the dye was applied to lysosomes.The results indicated that the dye has a significant ICT effect and has a good targeting effect in lysosomes.