A method for determination of heptafluorobutyryl chloride content by high performance liquid chromatography-evaporative light scattering was established.The chromatographic column HILIC C18(100×3.0,3.5 μm)was used for equal degree elution with methanol-5 mmol/L ammonium acetate-formic acid aqueous solution as the mobile phase.The flow rate was 0.5 mL/min and the col-umn temperature was 30℃.Evaporator and nebulizer temperature were set at 90℃and 80℃,respectively,and the gas flow rate was 1.0 mL/min.The results showed the detection limit of heptafluorobutyryl chloride was 3 μg/mL and the quantitative limit was 10 μg/mL;the RSD of test on heptafluorobutyryl chloride repeatability and precision were both less than 2％,and the linear relationship of calibration curves for heptafluorobutyryl chloride was good(R2＞0.999)in the mass concentration range of 10～500 μg/mL.A sim-ple,reproducible and reliable method was provided for the determination of heptafluorobutyryl chloride contents.
关键词
高效液相色谱/蒸发光散射/七氟丁酰氯/含量测定
Key words
high performance liquid chromatography/evaporative light scattering/heptafluorobutyryl chloride/content determination
维普
万方数据