三叶草锈病发生的生理特性研究
Study on the Physiological Characteristics of Trifolium pratense
董帅 1李琼芬 1岳宁波 1周敏 1韩继镕 1蒋兰兰2
作者信息
- 1. 贵州省黔南州农业科学研究院,贵州黔南 558000
- 2. 贵州农业职业学院,贵州 贵阳 551400
- 折叠
摘要
研究以感病和未感病的三叶草叶片为实验材料,利用考马斯亮蓝法和紫外吸收法测定 2 种三叶草叶片的可溶性蛋白含量,同时测定了 2 种叶片的丙二醛含量.结果表明,2 种可溶性蛋白测定的实验方法所测结果基本一致,可溶性蛋白含量均是未感病叶片高于感病叶片,未感病叶片可溶性蛋白含量为 39.98mg/g.感病叶片丙二醛含量低于未感病叶片,说明未感病叶片对锈病的耐受性及抗病性均高于感病叶片.因此,研究从生理特性上为三叶草抗病资源的利用和抗病品种培育奠定了基础.
Abstract
In this study,susceptible and non-susceptible Trifolium pratense leaves were used as experimental materials.The soluble protein contents of the two kinds of leaves were determined by the Coomassie blue stain-ing and the ultraviolet absorption spectrophotometry,and the malondialdehyde(MDA)contents were also deter-mined.It was shown that the results of the two soluble protein determination experimental methods were basi-cally consistent.The soluble protein content of non-susceptible leaves(39.98 mg/g)was higher than that of sus-ceptible leaves.The MDA content of susceptible leaves was lower than that of non-susceptible leaves,indicat-ing that the non-susceptible leaves were more tolerant and resistant to rust.Therefore,this study lays a physio-logical foundation for the utilization of disease-resistant resources and the cultivation of new disease-resistant varieties.
关键词
三叶草/锈病/可溶性蛋白/丙二醛Key words
Trifolium pratense/Rust disease/Soluble protein/Malondialde引用本文复制引用
出版年
2024
NETL
NSTL
万方数据