摘要
为探究铁、锰、锌配施对黄芪抗氧化酶及产量质量的影响,应用三因素四水平"3414"试验,测定并记录黄芪抗氧化酶活性及产量品质.结果发现,铁、锰、锌肥各5 g/m2的1 000倍液对黄芪抗氧化酶活性、叶绿素含量、产量及品质影响最佳.过氧化氢酶、过氧化物酶及超氧化物歧化酶活性最强,分别为457.04 U/g、4870.34 U/g、1616.24 U/g;叶绿素a、叶绿素b及内胡萝卜素含量最多分别为2.80 mg/g、24.61 mg/g、5.07 mg/g;产量为6 280.49 kg/hm2,增产率达64.63%;总灰分含量最少为2.09%,黄芪甲苷和毛蕊异黄酮葡萄糖苷最多分别为0.115 3%、0.038 9%,浸出物含量排第3位为34.32%.铁锰锌配施黄芪抗氧化酶活性及产量质量结果均优于对照.铁锰互作对其抗氧化酶及产量质量作用最佳;单独施用锌肥对其抗氧化酶及产量质量影响最佳.铁锰锌配施对其抗氧化酶及产量质量具有促进作用,但施肥浓度过高或过低有不同程度抑制作用.
Abstract
To explore the effects of combined application of iron,manganese,and zinc on antioxidant enzymes,yield,and quality of Astragalus membranaceus,the"3414"experiment with three factors and four levels was used to measure and record the antioxidant enzyme activity,yield and quality of Astragalus membranaceus.The 1000 fold solution of iron,manganese,and zinc fertilizers of 5 g/m2 each had the best effect on the antioxidant enzyme activity,chlorophyll content,yield,and quality of Astragalus membranaceus.The activities of catalase,peroxidase,and superoxide dismutase were the strongest,457.04 U/g,4 870.34 U/g,and 1 616.24 U/g,respectively;The maximum contents of chlorophyll a,chlorophyll b,and endogenous carotene were 2.80 mg/g,24.61 mg/g,and 5.07 mg/g,respectively;The yield was 6 280.49 kg/hm2,with an increase rate of 64.63%;The minimum total ash content is 2.09%,while the maximum astragaloside A and Calyx isoflavone glucoside are 0.115 3%and 0.038 9%,respectively.The third highest extract content is 34.32%.The antioxidant enzyme activity,yield and quality of Astragalus membranaceus treated with Fe-Mn-Zn were better than those of the control.The interaction of iron and manganese had the best effect on antioxidant enzymes,yield and quality;Applying zinc fertilizer alone had the best effect on antioxidant enzymes and yield and quality.The combined application of iron,manganese,and zinc can promote the antioxidant enzymes,yield,and quality of rice,but too high or too low fertilization concentration has different degrees of inhibition.
基金项目
甘肃省现代农业产业技术体系建设项目中药材产业体系加工与储藏岗位项目(GARS-ZYC-2)
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甘肃省主产大宗药材规范化栽培技术研究项目(070003)
NETL
NSTL
万方数据