Determination of Six Phenylethanoid Glycosides in Two Hosts of C.Deserticola by HPLC-QAMS
To establish HPLC-QAMS and determine echinoside,mulleinside,iso-mulleinside,cistanoside A,tubuloside A and 2'-acetyl-mulleinside in alcohol extracts of Haloxau-Cistanche and Cistanoside guadripennis.The HPLC method showed good linearity in their respective linear ranges(r≥0.999 5).RSD were all lower than 3.0%.The average recoveries were 98.616%～100.203%,and the RSDs were 1.645%～2.602%.Using Echinacoside as the internal reference,the RCFs of the other five components were calculated respectively as 0.883 9,1.003 8,0.751 0,0.843 0 and 0.851 5,The contents of 6 phenylethanolsides in two host species C.deserticola were calculated by RCF,and there was no significant difference between QAMS and ESM in the contents of 6 phenylethanolsides in two host species C.deserticola.The results showed that the established HPLC-QAMS method for the determination of 6 phenylethanolsides in C.deserticola alcohol extract was stable and reliable,could be used for its quality control and basic research on pharmacodynamics.
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