Plant isolated microspore culture has the functions of rapidly purifying genotype and improving embryogenesis and plant regeneration rate,and plays an important role in mutation breeding,molecular marker-assisted breeding,and genetic engineer.Its influencing factors are reviewed,involving explant pretreatment,microspore pretreatment,donor genotype,the density of microspores,pH of media,and components of media.Isolated microspore culture in tree is seldom studies and its technical system is backward.Therefore,the current backward situation of tree breeding may be improved if a lot of work of isolated microspore in tree is done.
NETL
NSTL
万方数据
维普
