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海南小黄姜脱毒快繁技术研究

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以海南小黄姜茎尖为材料,进行组培脱毒及离体快繁技术研究,结果表明,剥离茎尖(0.3~0.5 mm)分生组织培养,能有效脱除生姜烟草花叶病毒和黄瓜花叶病毒。筛选出适合丛生芽分化的培养基配方(MS+6-BA3.0 mg/L+NAA0.1 mg/L),实现了生姜增殖与生根诱导同步,增殖系数为8,将试管苗移栽到沙土+椰糠1:1的混合基质中炼苗,成活率最高为92%。
Virus Elimination and Rapid Propagation of Small Yellow Ginger (Zingiber officinale Rosc) in Hainan
In order to investigate virus elimination and micropropagation of ginger, we use small yellow ginger stem tissue as material. The results showed with stem tip (0.3~0.5 mm) culture, could effectively remove Tobacco mosaic virus and Cucumber mosaic virus of ginger, which showed the medium MS+6-BA3.0 mg/L+NAA0.1 mg/L were best for buds propagation, we accomplished the proliferation and root induction synchronised, the propagation coefficient were 8 , transplant to hardening in sand+coconut husk 1:1 mixing matrix had the best transplanting survival rate were 92%.

stem tipin vitro propagationplantlets from tissue culturesurvival rate

齐兰、朱红林、陈健晓、白翠云

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海南省农业科学院粮食作物研究所 海南海口 571100

茎尖 离体快繁 组培苗 成活率

海南省农业厅示范推广项目海南省星火产业带项目[No

琼科函2012561号]

2013

热带农业科学
中国热带农业科学院

热带农业科学

CSTPCD
影响因子:0.534
ISSN:1009-2196
年,卷(期):2013.(9)
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