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生物有机肥和EM菌剂对菜园连作土壤微生物的影响

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采用盆栽试验,连续种植3批菜心,研究生物有机肥和EM菌剂对连作9a和6a的菜园土壤微生物群落结构和功能多样性的影响.结果表明:与化肥处理相比,施用生物有机肥能够提高连作土壤中细菌、放线菌、丛枝菌根真菌和革兰氏阴性细菌(G-)的相对含量及G-/G+(革兰氏阴性细菌/革兰氏阳性细菌)比值,并降低土壤真菌的相对含量;随着种植批次的增加,土壤中G-、G+含量降低,G/G+比值上升,生物有机肥和EM菌剂配施化肥处理G-含量普遍高于化肥处理;与化肥处理相比,施用生物有机肥土壤微生物A WCD值、Simpson指数、Mc Intos指数均提高,土壤微生物活性、多样性和均一度提高.
Effects of Bio-organic Fertilizer and EM Microbial Agent on Microbes of Continuous Cropping Soil in Vegetables Garden
Vegetable Choi Sum was pot tried for three rounds of cropping to observe the effects of bio-organic fertilizer and EM microbial agent on microbial community structure and functional diversity of the soil in the vegetable garden for 9 and 6 years of continuous cropping.The results showed that the bio-organic fertilizer increased the relative contents of bacteria,actinomycetes,arbuscular mycorrhizal fungi and gram-negative bacteria,and the gram-negative bacteria/gram-positive bacteria ratio of continuous cropping soil,but reduced the relative content of fungi,as compared to the chemical fertilizer.With the more croppings the bio-fertilizer reduced the relative contents of gram-negative bacteria,gram-positive bacteria,increased the gram-negative bacteria/gram-positive bacteria ratio.The bio-organic fertilizer and the EM agents +chemical fertilizer treatment generally had a higher relative content of gram-negative bacteria than the chemical fertilizer treatment.Comparing with chemical fertilizer,the bio-fertilizer treatment had a higher average well color development (A WCD),Simpson index and Mclntosh index of soil microbes as well as soil microbial activity,diversity and uniformity than the chemical treatment.

bio-organic fertilizerEM microbial agentsoil microbescontinuous cropping obstacle

陈文、刘晓、孙光闻、刘厚诚、朱梦禹、陈日远

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华南农业大学园艺学院 广东广州510642

生物有机肥 EM菌剂 土壤微生物 连作障碍

现代农业产业技术体系专项资金广东省省级科技计划项目

CARS-25-C-042014A020208113

2017

热带农业科学
中国热带农业科学院

热带农业科学

CSTPCD
影响因子:0.534
ISSN:1009-2196
年,卷(期):2017.37(4)
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