首页|土田七幼嫩根茎的组培快繁

土田七幼嫩根茎的组培快繁

扫码查看
以土田七的幼嫩根茎为外植体,通过对其芽启动诱导、继代增殖及生根诱导培养基的筛选,建立了土田七幼嫩根茎的离体快繁体系.结果表明:土田七幼嫩根茎的最佳芽诱导培养基为MS+6-BA 3.0 mg/L+NAA 0.10 mg/L+3%蔗糖+卡拉胶粉9.5 g/L+10%椰汁;继代增殖最佳的培养基为MS+6-BA 5.0 mg/L+NAA 0.10 mg/L+3%蔗糖+卡拉胶粉9.5 g/L+10%椰汁;生根最佳的培养基为MS+NAA 0.10 mg/L+3%蔗糖+卡拉胶粉9.5 g/L+10%香蕉泥+活性碳1.0 g/L;组培苗移栽较合适的基质为进口泥炭:红壤:腐叶土混合的基质.
Rapid Propagation of Stahlianthus involucratus via in Vitro Culture of Young Rhizomes
Young rhizomes of Stahlianthus involucratus were used as explants for tissue culture.Media for bud inducing, subculture and rooting were screened to establish a rapid propagation system based on young rhizomes of S. involucratus. The results showed that the optimum medium was MS+6-BA 3.0 mg/L+NAA 0.10 mg/L+3% sucrose+carrageenan powder 9.5 g/L+10% coconut water for inducing of adventitious buds, MS+6-BA 5.0 mg/L+NAA 0.10 mg/L+3% sucrose+carrageenan powder 9.5 g/L+10% coconut water for subculture, and MS+NAA 0.10 mg/L+3% sucrose+carrageenan powder 9.5 g/L+10% mashed banana + activated carbon 1.0 g/L for rooting. The optimum medium for transplanting of the rooted plantlets was the mixture of imported peat,red soil and humus soil.

Stahlianthus involucratusStahlianthusrhizomepropagation

李冬梅、赵超艺、刘小飞

展开 >

广东省农业科学院环境园艺研究所 广东广州510640

土田七 土田七属 根茎 繁殖

广东省科技计划项目广东省科技计划项目广州市科技计划项目

2015A0202090782015B070701016201607010101

2017

热带农业科学
中国热带农业科学院

热带农业科学

CSTPCD
影响因子:0.534
ISSN:1009-2196
年,卷(期):2017.37(10)
  • 2
  • 12