Screening of Phosphorus-solubilizing Bacteria in Sugarcane Rhizosphere Soil and the Optimization of Culture Conditions
Two phosphate-solubilizing bacteria (N1-1-3 and H4-3-1) with strong phosphate-dissolving ability were screened out using P-solubilizing circle method and the Mo-Sn – Vc method from the rhizosphere soil of sugarcane, and the culture conditions were optimized by single factor test and multivariate orthogonal test. The results showed that the optimum culture conditions was as follows when lactose was used as carbon source and NH4Cl as nitrogen source, the initial pH 6.5 and the culture temperature 30℃ for the strain N1-1-3, and glucose as carbon source, KNO3 as nitrogen source, the initial pH 7.5 and the culture temperature 30℃ for the strain H4-3-1. Under the optimum culture conditions, the amount of dissolved phosphorus was 190.57 mg/L and 167.82 mg/L, respectively. N1-1-3 and H4-3-1 phosphate-solubilizing strains had strong ability to dissolve phosphorus and could be used as pot application test or field test application material.
phosphate bacteriasugarcanerhizosphere soiloptimization of culture conditions
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