首页|SRAP技术分析海南降香檀遗传多样性

SRAP技术分析海南降香檀遗传多样性

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为探讨海南降香檀遗传多样性,以采自海南省东、西、南、北、中五个区域23个点185份降香檀单株材料为材料,采用SRAP分子标记对其遗传多样性进行分析.结果表明:利用筛选出的25对SRAP引物组合,扩增共检测到174个位点,多态性位点92个,多态率为52.8%.UPGMA聚类分析结果表明,185份海南降香檀种质材料与对照多裂黄檀遗传相似系数为0.56;在相似系数约为0.73处,可将收集到降香檀种质划分为4个类群.SRAP标记可较好地反映海南降香檀种质间的遗传多样性,为合理保护和利用海南降香檀种质资源提供科学依据与借鉴,并为海南降香檀分类系统和进化关系提供分子系统学依据.
Analysis of Genetic Diversity of Dalbergia odorifera Growing in Hainan by SRAP
In order to explore the genetic diversity of Dalbergia odorifera in Hainan, 185 individual trees of D. odorifera collected from 23 locations in the eastern, western, southern, northern and central regions of Hainan Province were collected for analysis of their genetic diversity by using a molecular marker system, SRAP. The results showed that a total of 174 sites were detected by using the 25 pairs of SRAP primers screened previously, and that their polymorphic sites were 92, with a polymorphism rate of 52.8%.The UPGMA cluster analysis showed that the genetic similarity coefficient between the 185 individual trees of D. odorifera collected in Hainan and Dalbergia rimosa Roxb was 0.56. When the similarity coefficient was 0.73, the accessions of D. odorifera germplasm collected in Hainan could be divided into four groups. The SRAP molecular marker system could reveal the genetic diversity of D. odorifera growing in Hainan. This study provided a molecular systematic basis for the classification system and evolutionary relationship, and for the protection and rational utilization of the D. odorifera resources in Hainan.

SRAPDalbergia odoriferagenetic diversity

孟慧、陈波、杨云

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中国医学科学院﹠北京协和医学院, 北京 100193

药用植物研究所海南分所/海南省南药资源保护与开发重点实验室, 海南海口 570311

SRAP 降香檀 遗传多样性

中国医学科学院医学与健康科技创新工程项目

2016-I2M-2-003

2018

热带农业科学
中国热带农业科学院

热带农业科学

CSTPCD
影响因子:0.534
ISSN:1009-2196
年,卷(期):2018.38(12)
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