Screening,Identification,and Evaluation of the Antagonistic Effects of Deep-sea Fungi for Biocontrol of Rubber Red Root Disease
Rubber root rot is one of the devastating diseases in China's tropical rubber industry that seriously affects the de-velopment of the rubber industry and the restoration of tropical artificial forest ecosystems.It is urgent to find an efficient and safe control measure to overcome the shortcomings of physical and chemical control in practical production.With the devel-opment of science and technology and the increasing demand for environmental protection,biological control has become the most promising research direction for pest control.This study targeted the pathogenic bacteria of rubber root rot,used deep-sea sediment culturable fungi as indicator fungi,and screened for initial activity using the plate confrontation method to obtain biocontrol fungi with high antagonistic activity against the pathogenic bacteria of rubber root rot.The antagonistic ef-fects of the active strains were further evaluated through plate confrontation,hole plate,and paper disk methods to measure the inhibitory effects of fermentation broth,volatile metabolites,and nonvolatile metabolites,respectively.The results showed that among the 56 strains of deep-sea fungi tested,nine strains exhibited activity in the plate confrontation assay.The results of the secondary screening showed that the inhibition rates of the fermentation broth,volatile metabolites,and nonvolatile metabo-lites of the nine fungal strains against the pathogenic bacteria ranged from-29.55％to 48.45％,-3.56％to 51.46％,and-10.00％to 50.00％,respectively.Among them,the inhibition rates of the fermentation broth,volatile metabolites,and non-volatile metabolites of the strains DSF034 and DSF073 against the pathogenic bacteria were significantly greater than those of the other strains.The inhibition rates were 21.69％,32.04％,and 33.33％for DSF034,and 48.45％,51.46％,and 50.00％for DSF073,respectively.Based on morphology and rDNA-ITS sequence analysis,the strains DSF034 and DSF073 were identi-fied as Penicillin sp.and Aspergillus sp.,respectively.These two strains showed good potential for use in biocontrol and could provide new resources for the selection of biocontrol agents for rubber root rot.
rubber red root diseasebiological controldeep-sea fungiantagonism
万方数据
维普
