Analysis of the Chemical Constituents of the Eucalyptus cloeziana Bark and Research on Its Antioxidative Activities
The purpose of this study was to rapidly analyze and identify the chemical constituents of Eucalyptus cloeziana bark extract using ultra high performance liquid chromatography-quadrupole/exactive Orbitrap mass spectrometry(UPLC-Q-EXACTIVE-MS)and determine the vitro antioxidant activity of these compounds to provide a scientific basis for the exploi-tation of their bark resources.A Waters ACQUITY UPLC BEH C18 column(2.1 mm×50 mm×1.7 μm)was used for gradient elution with 0.1%formic acid aqueous solution and methanol as the mobile phase at a 3.0 mL/min flow rate.The electrospray ion source(ESI)was used for mass spectrometry data in positive and negative ion modes.According to the accurate excimer ion peak and secondary mass spectral information provided by UPLC-Q-EXACTIVE-MS,a total of 26 compounds in Euca-lyptus cloeziana bark were identified or tentatively identified by conjunction with the relevant literature,which included two flavonoids,15 organic acids,one triterpene,two alkaloids,and six fatty acid amides.The in vitro antioxidant test results showed that the bark extract of E.cloeziana had good DPPH and ABTS scavenging abilities,with IC50 values of 60.02 µg/mL and 76.94 µg/mL,respectively.This study is the first to establish that the UPLC-Q-EXACTIVE-MS method is a rapid,accu-rate,and comprehensive method for identifying the chemical composition of the bark of E.cloeziana,and the resulting cleav-age pattern can provide a reference for the identification of other similar chemical components.However,E.cloeziana bark has good antioxidant activity and can be a natural source of active antioxidant substances.
万方数据
维普
