首页|激素浓度组合对成熟油梨枝条伸长和生根的影响

激素浓度组合对成熟油梨枝条伸长和生根的影响

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为了建立抗根腐病成熟油梨砧木的快繁技术体系,通过设置不同浓度(0.1、0.5、1.0、1.5、2.0、2.5、3.0 mg/L)的细胞分裂素(meta-topolin,MT)与不同浓度(0.1、0.5、1.0、1.5 mg/L)的赤霉素(gibberellin,GA3)的组合试验,研究适宜于成熟油梨外植体伸长的培养条件;同时设置不同浓度(0.5、1.0、1.5、2.0、2.5、3.0 mg/L)的吲哚-3-丁酸(indole-3-butyric acid,IBA)的生根培养基,探究最适油梨外植体生根的培养条件.研究发现,伸长试验 30 d时(D30)油梨外植体株高增量和茎粗增量的效应量依次是MT浓度×GA3 浓度>MT浓度>GA3 浓度;对D30 叶片增量的效应量依次是 MT 浓度>MT 浓度×GA3 浓度>GA3 浓度.结果表明,在该研究的测试范围内,最适于伸长的激素浓度组合是 MT 2 mg/L、GA3(0.5~1.0)mg/L,而在测试的 IBA 浓度范围内均没有观察到根系的生长.通过显微结构观察发现,MT浓度为 1 mg/L处理的油梨外植体茎木质部(木质部厚度为 171.6 μm)生长优于MT浓度为 0.1 mg/L的处理(木质部厚度为 95.3 μm),这对后期的成功驯化有一定积极作用.本研究结果可为成熟油梨快繁体系建立提供借鉴.
Effects of Hormone Concentration Combinations on Branch Elongation and Rooting in Mature Avocado
This experiment was conducted to investigate the suitable culture conditions for the elongation of mature oil pear explants by combining cytokinin(meta-tooling,MT)at different concentrations(0.1,0.5,1.0,1.0,and 1.5 mg·L-1)and gib-berellin(GA3)at various concentrations(0.1,0.5,1.0,and 1.5 mg·L-1)to study the culture conditions suitable for the elonga-tion of mature avocado explants to establish a fast breeding technology system for root rot-resistant mature oil pear rootstocks,and at the same time,rooting media with different concentrations(0.5,1.0,1.5,2.0,2.5,and 3.0 mg·L-1)of indole-3-butyric acid(IBA)were used to determine the optimal culture conditions for the rooting of avocado explants.We investigated the most suitable culture conditions for the rooting of avocado explants.The effects of the elongation test for 30 d(D30)on the increase in plant height and stem thickness of avocado explants were in the order of MT concentration×GA3 concentration>MT con-centration>GA3 concentration,and the effects on the increase in leaf blades of D30 were in the order of MT concentration>MT concentration×GA3 concentration>GA3 concentration.The most suitable hormone concentration combinations for elongation in the tested range of this study were 2 mg·L-1 MT and 0.5-1.0 mg·L-1 GA3,and no root growth was observed in any of the tested IBA concentration ranges.Microstructural observation revealed that the stem xylem of oil pear explants treated with an MT concentration of 1 mg·L-1(with a xylem thickness of 171.6 μm)grew better than that of plants treated with an MT con-centration of 0.1 mg·L-1(with a xylem thickness of 95.3 μm),indicating that MT had a positive effect on successful domesti-cation at a later stage.The results of this study can be helpful for future fast propagation systems for mature avocado estab-lishment.

mature avocadohormoneroot rot resistant rootstockelongationrootingacclimatization

成镜、汤倩、黄天带、顾晓川、徐正伟、彭素娜

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海南天然橡胶与热带林木研究院 海南海口 571101

中国热带农业科学院橡胶研究所/农业农村部橡胶树生物学与遗传资源利用重点实验室/省部共建国家重点实验室培育基地/海南省热带作物栽培生理学重点实验室/热带林木种子种苗工程中心/海口市热带植物种苗创新重点实验室 海南海口 571101

海南大学林学院 海南儋州 571737

成熟油梨 激素 抗根腐病砧木 伸长 生根 驯化

海南省自然科学基金项目海南省优秀团队"橡胶遗传育种"专项资金

321QN34920210203

2024

热带农业科学
中国热带农业科学院

热带农业科学

CSTPCD
影响因子:0.534
ISSN:1009-2196
年,卷(期):2024.44(3)
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