摘要
针对常规石蜡切片方法无法获得完整桑树花芽切片的问题,以"无籽大十"品种桑树花芽为供试材料,在常规石蜡切片技术基础上,对石蜡切片样品制作过程中固定、软化、脱水、透明及染色等环节进行了优化.结果表明,使用 15%氢氟酸软化后,采用优化后的系列脱水、透明等步骤进行制片,可以使切片连续性、完整性得到明显提高.通过切片显微观察,可见细胞轮廓明显、裂痕较少、结构较完整,染色较清晰,并且对发育后期的花芽效果更为明显.优化后的制样流程解决了常规制片出现浸蜡不彻底、组织变脆、蜡带不连续、内部结构不完整等一系列问题.该研究可以从解剖学上为桑树花芽发育形态和花芽分化机制研究提供技术参考.
Abstract
To address the inability of the conventional paraffin sectioning method to obtain complete sections of mulberry flower buds,this study used"seedless 10"mulberry flower buds as test materials.Based on the conventional paraffin section technique,we optimized the fixation,softening,dehydration,transparency,and dyeing of paraffin section samples.The results show that after softening with 15%hydrofluoric acid,the continuity and integrity of the slices can be improved by adopting the optimized series of dehydration and transparency steps.Through microscopic observation,it can be seen that the cell out-line is distinct,the cracks are few,the structure is complete,the staining is clear,and the effect on flower buds in the later stage of development is more distinct.The optimized sample preparation process has solved problems such as incomplete wax soaking,brittle structures,discontinuous wax bands,and incomplete internal structures in conventional production.This study provides a technical reference for the study of mulberry flower bud development morphology and flower bud differentiation mechanisms from an anatomical perspective.
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