An Improved Method for Paraffin Sections of Mulberry Flower Buds
To address the inability of the conventional paraffin sectioning method to obtain complete sections of mulberry flower buds,this study used"seedless 10"mulberry flower buds as test materials.Based on the conventional paraffin section technique,we optimized the fixation,softening,dehydration,transparency,and dyeing of paraffin section samples.The results show that after softening with 15%hydrofluoric acid,the continuity and integrity of the slices can be improved by adopting the optimized series of dehydration and transparency steps.Through microscopic observation,it can be seen that the cell out-line is distinct,the cracks are few,the structure is complete,the staining is clear,and the effect on flower buds in the later stage of development is more distinct.The optimized sample preparation process has solved problems such as incomplete wax soaking,brittle structures,discontinuous wax bands,and incomplete internal structures in conventional production.This study provides a technical reference for the study of mulberry flower bud development morphology and flower bud differentiation mechanisms from an anatomical perspective.
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