Passiflora caerulea L.Interspecific Hybrid F1 Progeny Molecular Identification and Genetic Diversity Analysis
Interspecific hybridization was carried out with the red flower Passiflora as the mother and the large fruit Passi-flora as the father.Through the observation and investigation of the botanical characteristics of the hybrid F1 generation population,five pairs of SRAP primers with stable amplification results and polymorphisms were selected to identify the F1 hybrid generation population.The rule of gene separation,genetic diversity,and genetic relationships between the parents and the hybrid F1 generation population were analyzed.The results showed that the vine shape,nectary position,and leaf shape of 29 single plants in the F1 generation were separated,indicating different degrees of diversity.The results of SRAP molecular identification showed that five pairs of SRAP primers could be used to identify 29 single hybrid strains,illustrating that these single strains were true hybrids.The UPGMA method was used to construct the system cluster graph.The results showed that at a genetic similarity coefficient of 0.60,the parents and 29 hybrid F1 generation plants could be divided into three types,among which the first type was grouped with the mother"hh"and a total of 12 hybrid F1 generation plants,the second type was grouped with the father"dg"and a total of 11 hybrid F1 generation plants,and the third type had six hybrid F1 generation plants.The results showed that the SRAP could be used to identify the F1 hybrids of Passiflora.
万方数据
维普
