Effects of Light on the Growth and Physiology of Three Strains of Polygonati rhizoma Seedlings
To determine the required light intensity for the robust growth of Polygonatum sibiricum tissue-culture-generated plants,three strains of P.sibiricum tissue-culture-generated plants,26,83,and 91,were used as experimental materials.With 2 000 lx as the control,4 000,6 000,8 000,10 000,20 000 lx light intensities were used to measure the growth and physio-logical indices of the plants.The results showed that the effect of light on the growth of different strains of tissue-cultured plants differed.When the light intensity was 6 000 lx,the growth of the tissue-cultured plants was the greatest,with shorter root length pairs and the 26 and 91 strains had the greatest number of roots.The fresh weight ratio of the aboveground and underground parts of strain 83 first increased and then decreased.The drying rate of the three strains of tissue-cultured seed-lings was significantly affected by the light(p<0.01),and the drying rate significantly increased when the light intensity was greater than 8 000 lx.With increasing light intensity,the superoxide dismutase(SOD)and peroxidase(POD)enzyme activities of tissue-cultured seedlings first increased and then decreased.The soluble protein content tended to increase,decrease,and then increase.With increasing light intensity,the chlorophyll content of strain 91 showed a single peak trend,with the highest chlorophyll(a+b)content occurring at a light intensity of 6 000 lx;those of 26 strain show a"V"shaped trend,with the high-est content at a light intensity of 20 000 lx;and those of 83 strains showed a tilted"N"shaped trend of change,and the content was greatest when the illumination reached 20 000 lx.When the illumination reached 6 000 lx,the quality of P.sibiricum seed-lings improved;26 P.sibiricum strains exhibited strong adaptability in high-light environments.
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