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正交试验优化铁皮石斛传统种苗繁育技术

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为了提高铁皮石斛传统育苗腋芽萌发成苗率,指导铁皮石斛传统育苗技术在生产上的推广应用,开展影响腋芽萌发成苗率因子[繁育模式(A)、激素处理(B)、育苗基质(C)、品系(D)]的四因素五水平正交试验研究.结果显示:影响传统育苗腋芽萌发成苗率因素主次顺序为D>A>B>C,4 个因素的最优水平分别是A2(整条平铺并覆盖基质1 cm左右)、B2(NAA 0.5 mg/L+6-BA 0.5 mg/L)、C1(苔鲜)、D4(紫精灵),试验筛选出传统育苗因素最优组合A2B2C1D4,其萌发成苗率可达 81%,较自然腋芽萌发成苗率(10%~20%)大大提高.
Optimization of Traditional Seedling Breeding Methods for Dendrobium officinale via Orthogonal Experiments
To improve the germination rate of axillary buds in traditional Dendrobium officinale seedlings and guide the promotion and application of traditional seedling cultivation techniques in production.,a study was conducted on the factors influencing germination and seedling rates of traditional seedling-raising axillary buds.A four-factor and five-level orthogonal experiment was designed for the breeding mode(A),hormone treatment(B),seedling substrate(C),and strains(D).The results revealed that the prime and secondary order of factors affecting the germination and seedling rate of axillary buds in traditional seedling cultivation was D>A>B>C.The optimal levels of the four factors are A2(the whole strip is laid flat and covered with a substrate for approximately 1 cm),B2(NAA 0.5 mg·L-1+6-BA 0.5 mg·L-1),C1(fresh moss),D4(purple sprite),and the optimal combination of traditional seedling cultivation factors A2B2C1D4 was selected through experiments,and its germination and seedling rates can reach 81%,which is significantly higher than the original natural axillary bud germination and seedling rates of 10%~20%.

orthogonal experimentDendrobium officinaleseedlings growthbreeding

张志勇、周美玲、郑新妹、张川、梁金平

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龙岩市农业科学研究所 福建龙岩 364000

正交试验 铁皮石斛 育苗 繁育

福建省科技计划项目——引导性项目福建省科技计划项目——引导性项目

2021N00582023N0058

2024

热带农业科学
中国热带农业科学院

热带农业科学

CSTPCD
影响因子:0.534
ISSN:1009-2196
年,卷(期):2024.44(6)