Effects of a Biochar-based Fertilizer on the Leaf Water Potential of Lespedeza bicolor Seedlings under Drought Stress and Rewatering
To research the response characteristics of the leaf water potential of Lespedeza bicolor seedlings under drought stress and rewatering,various amounts of biochar-based fertilizer have been applied.Pot experiments were conducted in a greenhouse to estimate the effects of different application rates of biochar-based fertilizer(750,1 500,and 2 250 kg·hm-2),various levels of water stress(100.00%,87.84%,70.00%,52.16%,and 40.00%soil relative water content),and rewatering conditions on the L.bicolor seedling leaf water potential.The results revealed that severe drought conditions led to a decrease in leaf water potential and an increase in the water stress index of L.bicolor seedlings when biochar-based fertilizer was ap-plied at rates of 750,1 500,and 2 250 kg·hm-2.Among the treatments,the lowest leaf water potential in seedlings was ob-served under the 40.00%SRWC treatment.In particular,applying three fertilizer levels(750,1 500,and 2 250 kg·hm-2)re-sulted in a decrease of 0.69 MPa and 0.48 MPa in the average leaf water potential after 15 d and 30 d of drought stress,re-spectively.However,the leaf water potential of L.bicolor seedlings exhibited recovery over time upon rewatering.A strong correlation was found between the severity of drought stress and the duration of the drought.In contrast,after six days of re-watering,the leaf water potential of L.bicolor seedlings subjected to 15 days of drought stress did not fully recover to the control level in the 52.16%and 40.00%SRWC treatments.However,the leaf water potential of L.bicolor seedlings in the other treatments recovered to the control level and even exhibited compensatory and super-compensatory effects.