Preliminary Identification of Beetle Species in Litchi Orchards in Yunnan Based on Sanger Sequencing Technology
As one of the crucial industries for rural revitalization in Yunnan Province,litchi has diversified its planting eco-logical environment with the continuous expansion of its area,and the types of diseases and pests have also increased.Beetles have been discovered in the main production areas of litchis in Yunnan in recent years.Investigating the harm caused by litchi beetles and identifying their types is highly important for the prevention and control of lychee diseases and pests in Yunnan.This study adopted a 5-point sampling method.First,a survey was conducted on the degree and status of damage caused by beetles in the five main production areas of litchi in Yunnan.Sanger sequencing technology was used for preliminary identifi-cation of physiological races.There were varying degrees of damage caused by beetles in all five production areas,and the damage pattern was similar to that of insects in the family Scorpaenidae.After Sanger sequencing technology was used for detection,a preliminary identified through comparison in the NCBI database revealed that there are two genera and three spe-cies,namely Sinotylon perferans,Sinoxylon manage,and Bostrichus capuchinus.
litchiSanger sequencingSinoxylon analeSinotylon perforanspest control
万方数据
维普
