To investigate the effects of different factors on the cutting of the'Black Diamond'Syzygium samarangense Merr.et Perry.under sugar-free culture conditions,and select the optimal sugar-free cutting combination,providing a theoretical basis for the cutting and seedling cultivation of lotus mist.This study utilized sugar-free tissue culture equipment,using'Black Diamond'S.samarangense Merr.et Perry.branches as cuttings and conducted orthogonal experiments at four factors and three levels:nutrient solution type(A),branch type(B),rooting agent ABT solution concentration(C),and soaking time(D).Fur-ther optimization experiments were conducted on this basis.The results revealed that the factors influencing the rooting effect of lotus mist in the orthogonal experiments were A>D>C>B,and the optimal combination was A1B3C1D2.The factors affecting the rooting rate were B>A>D>C,and the optimal combination was A1B2C1D2.The influencing factors of the decay rate are C>A>D>B,and the optimal combination is A3B1C1D2.The optimization experiment used MS(sugar-free)nutrient solution and vermiculite as the cutting substrate.When soaked in ABT solution at concentrations of 150 or 250 mg.L‒1 for 2 minutes,the cutting effect indices were 2.90 and 2.83,respectively.This treatment is conducive to rooting the branches of'Black Dia-mond'Syzygium samarangense Merr.et Perry.and the cutting effect is good.
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