Optimization of the Banana Genetic Transformation System
The highly sterile and polyploid characteristics of banana(Musa spp.)severely hinder the application of conven-tional breeding methods.However,banana genetic transformation technology suffers from strong genotype dependence and low transformation efficiency.In this study,Agrobacterium tumefaciens was utilized to mediate the transformation of the GUS gene,and multiple bud clumps of the Musa acuminata cv.Mas(AA)were used as the starting transformation material.To optimize the genetic transformation conditions by studying the effects of the transformation status of the explants,different transformation vectors and incubation times of acetosyringone(AS),concentrations of Agrobacterium tumefaciens,pre-cultivation times,different Agrobacterium strains and co-cultivation methods on the process of banana genetic transfor-mation were studied,to obtain a highly efficient genetic transformation system for banana.The results revealed that the multi-ple bud slices of M.acuminata cv.Mas(AA)were used as explants and were pre-cultured for 1 d.When the infiltration solu-tion was prepared by using Agrobacterium EHA105 containing the plant expression vector constructed with p CAMBIA1304,the AS was incubated for 0-4 h,and the OD600 of A.tumefaciens infestation solution was 0.4-0.6.The infiltrated multiple bud slices were placed in a solid MS media(with the addition of 200 μmol·L-1 AS)for co-culture,and the transformation effi-ciency of regenerated plants was better.Optimizing the conditions of the genetic transformation system to improve the genetic transformation rate of bananas will provide vital technical support for solving the crucial technical problems in the develop-ment of the banana industry and the precisely directed molecular breeding of bananas.It will provide a critical guarantee for the healthy and sustainable development of the banana industry.
NETL
NSTL
万方数据
