Molecular typing and drug resistance of Salmonella from diarrheal patients in Guangzhou
Objective To understand the molecular types and drug resistance of Salmonella isolated from diarrhea cases in Guangzhou during 2019 and provide scientific basis for early prevention,control and tracing of Salmonella infection.Methods The serotypes,drug sensitivity and pulse-field gel electrophoresis(PFGE)type of all the stains of Salmonella isolated from 7 designated hospitals were detected.Homology analysis was performed using the comparison function of the Chinese Pathogen Identification Net.Results A total of 303 strains with accurate serotype detection were collected,of which 56.44％(171/303)were Salmonella;Salmonella 1,4,[5],12∶i:-was the dominant serotype.Other serotypes with higher proportions were S.enteritidis 11.22％(34/303),S.stanley 4.29％(13/303)and S.London 3.96％(12/303).Through antibiotic resistance test,it was found that the highest resistance were ampicillin(87.79％)and tetracycline(87.13％),and 90.76％of the strains were resistant to more than 3 antibiotics.PFGE cluster analysis showed that Salmonella 1,4,[5],12∶i:-isolates were significant differences between each other,with a similarity of 42.97％-100.00％.There were few differences among S.enteritidis,and the similarity was 75.05％-100.00％.Conclusions Salmonella 1,4,[5],12∶i:-was the dominant serotype causing infectious diarrhea in Guangzhou city.The proportion of multi-drug-resistant(MDR)strains was high.PFGE types were diverse,and the genetic similarities of strains of different serotypes varied greatly.
NETL
NSTL
万方数据
