Active TMEM206 channel enhances apoptosis in neuroblastoma cells
Objective To explore the effect of the proton-activated Cl-(PAC)channel composed of transmembrane protein 206(TMEM206)on apoptosis in neuroblastoma cells under acidic conditions.Methods The TMEM206 gene was silenced using short hairpin RNA technology in neuroblastoma cells.Apoptosis in neuroblastoma cells was investigated using flow cytometry under neutral 7.2 and acidic 5.0 solution.Results The results showed that the apoptotic rate was 12.52％in neuroblastoma cells and 10.95％in the TMEM206 gene silenced neuroblastoma cells under neutral 7.2 solution.The change in the apoptosis rate did not reach statistical difference between neuroblastoma cells and the TMEM206 gene silenced neuroblastoma cells under neutral 7.2 solution(x2=0.53,P＞0.05).Our study also indicated that the apoptotic rate was 53.11％in neuroblastoma cells and 37.00％in the TMEM206 gene silenced neuroblastoma cells under acidic 5.0 solution.The apoptosis rate was significantly increased in neuroblastoma cells treated at pH 5.0 was higher than that of cells treated at pH 7.2(12.52％vs.53.11％,x2=12.26,P＜0.01).The TMEM206 gene silencing decreased the apoptosis rate of neuroblastoma cells under acidic 5.0 solution(53.11％vs.37.00％,x2=3.85,P＜0.05).Conclusion The TMEM206 gene silencing could protect acidosis-induced cell apoptosis in neuroblastoma cells.
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