Circumsporozoite protein gene polymorphism of imported Plasmodium vivax in Hubei province
Objective To analyze the genetic polymorphisms of Plasmodium vivax circumsporozoite protein(Pvcsp)in imported P.vivax malaria cases in Hubei province.Methods Epidemiological data and blood samples were collected from the imported P.vivax malaria cases in Hubei province in the China information system for disease control and prevention from 2017 to 2021.Genomic DNA of Plasmodium was extracted from blood samples and Pvcsp were amplified by nested PCR.After electrophoresis analysis by Qsep 100 automatic nucleic acid protein analyzer,the amplification products were digested with restriction endonuclease and genetic polymorphism was investigated with PCR-RFLP.Results A total of 46 blood samples of imported P.vivax malaria cases were analyzed,and all samples were successfully amplified to produce around 700 bp target bands,including 26 cases from Africa,12 cases from South Asian,6 cases from Southeast Asian,1 case from South America and 1 case from Oceania.The PCR fragments obtained were divided into bins that differed by 20 bp.Seven distinguishable Pvcsp size variants(designated A to G)with a size range of 664-795 bp,and variant A accounted for 34.78％(16/46).The number of size variants in cases imported from Africa,Pakistan and Southeast were seven,four and five,respectively,and there was no significant difference in fragment size variants types between Africa and Asia(x2=6.546,P=0.363).PCR-RFLP analysis of Pvcsp revealed that the parasites were VK210 type(97.83％,45/46)and VK247 type(2.17％,1/46,imported from Indonesia).Conclusion The Pvcsp gene of the imported P.vivax malaria cases reported in Hubei during 2017-2021 were characterized being highly polymorphic,and VK210 repeat type was the dominant genotype.
Plasmodium vivaxImportedCircumsporozoite protein genePolymorphism
NETL
NSTL
万方数据
