Expression of PD-1 in CD4+T cells in the lung of Plasmodium infected mice and its functional characteristics
Objective To investigate the expression and function of programmed cell death 1(PD-1)on CD4+T cells in lung of mice infected with Plasmodium.Methods Female C57BU6 mice from 5-6 weeks were randomly divided into normal or infected groups.Flow cytometry was used to detect the expression of PD-1 on CD4+T cells with Plasmodium infection,and the phenotype and cytokine secreting ability of CD4+T cells expressing PD-1 was detected by flow cytometry.qRT-PCR was used to detect the expression of related transcription factors B lymphocyte-induced maturation protein 1(Blimp-1),nuclear factor-activated T cell 1(NFATc1),signal transducer and activator of transcription(STAT)3,STAT4,interferon-stimulated gene factor 3(ISGF3),activator protein 1(AP-1),Notch,forkhead box transcription factor 1(FoxO1),nuclear factor-KB(NF-KB).Results After infection with Plasmodium,the proportion of CD4+T cells expressing PD-1 in the lungs of mice was increased significantly from 11.66％to 58.60％,the difference was statistically significant(t=7.892,P＜0.01).In the infected group,PD-1+CD4+T cells expressed more CD69 than PD-1CD4+T cells,which showed a statistically significant difference(t=9.856,P＜0.01).In the infected group,compared with PD-1CD4+T cells,more PD-1+CD4+T cells secreted IFN-γ,interleukin(IL)-2 and IL-10,the differences were statistically significant(t=7.301,4.876,7.607;all P＜0.05).QRT-PCR showed that compared with the normal group,the expression level of various transcription factors was changed in the infected group,which showed a statistically significant difference(all P＜0.05).Conclusion After infected with Plasmodium,CD4+T cells expressed PD-1 in the lung of mice were involved in the immune response,and played a significant role in immunomodulatory through secreting cytokines.
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