Effect of magnetic nano-Fe3O4 particles on Echinococcus granulosus-infected mice
Objective To investigate the effect of magnetic nano-Fe3O4 particles(MNPs-Fe3O4)on Echinococcus granulosus-infected mice.Methods A total of 18 E.granulosus-infected BALB/C mice were selected and randomly divided into 2 groups of 9 mice each,into control and MNPs-Fe3O4 groups.The mice in the control group were injected with saline in the tail vein,and the mice in the MNPs-Fe3O4 group were injected with 10 mg/kg MNPs-Fe3O4 in the tail vein,and the mice were administered continuously for 5 days per week.After 5 weeks of administration,the mice were anesthetized and dissected,and the lesions and tissues were collected to analyze and compare the wet weight of the cysts and the number of cysts in the control group and the MNPs-Fe3O4 group,and the liver,kidney,brain,spleen and lung tissues were stained with hematoxylin-eosin(HE)to observe the pathological structural changes.Results The particle size of MNPs-Fe3O4 was 150-160 nm.The differences in cyst wet weight and cyst number between control group and MNPs-Fe3O4 group mice were not statistically significant(t=-0.011,-0.406;all P＞0.05).HE staining showed that MNPs-Fe3O4 produced accumulation in the form of magnetic particles in the organs of liver,lung,spleen and kidney,but there was no inflammatory reaction.Conclusion MNPs-Fe3O4 had no therapeutic effect on E.granulosus-infected mice,but could be used as a potential cystic echinococcosis(CE)therapeutic drug carrier,and this study provides a theoretical basis for the development of MNPs-Fe3O4-loaded CE therapeutic drugs.
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