Expression of LAG-3 in CD4+T cells in patients with active pulmonary tuberculosis and the effect of LAG-3 on the function of CD4+T cells
Objective To investigate the expression of lymphocyte activation gene-3(LAG-3)in CD4+T cells in patients with active pulmonary tuberculosis and the effect of LAG-3 on the function of CD4+T cells.Methods 25 patients with active pulmonary tuberculosis and 20 healthy controls were recruited from the Infectious Diseases Hospital Affiliated to Soochow University between May 2020 and May 2021,used as the case group and the control group,respectively.The expression of LAG-3 on CD4+T cells in peripheral blood and bronchoalveolar lavage fluid were detected by Flow cytometry.Peripheral blood mononuclear cells(PBMC)of tuberculosis patients was stimulated with Mycobacterium tuberculosis specific antigen early secreted antigenic target 6 000 Mr and culture filtrate protein 10 000 Mr(ESAT-6/CFP10)in vitro to explore the expression of LAG-3 on tuberculosis specific CD4+T cells.Divided into the ESAT-6/CFP10 group and the Control group without the ESAT-6/CFP1O protein.The effects of LAG-3 on CD4+T cell functions were investigated by inhibition of solubility LAG-3(sLAG-3)protein(sLAG-3 group).Results The percentage of LAG-3+CD4+T cells in PBMC and bronchoalveolar lavage fluid(BALF)increased and decreased after treatment,the differences were statistically significant(t=2.291,3.045;all P＜0.05).After stimulated by ESAT-6/CFP10,compared with the Control group,the expression of LAG-3 in ESAT-6/CFP10 group was up-regulated on CD4+T cells,while the expression of interferon-γ(IFN-γ)was down-regulated,the differences were statistically significant(t=6.174,3.476;all P＜0.05).After inhibition of LAG-3 molecular signaling,compared with the ESAT-6/CFP10 group,the expression of IFN-γ and Granzyme B was elevated on CD4+T cells in the sLAG-3 group,the differences were statistically significant(t=3.882,3.205,P=0.178,0.033).Conclusions The expression of LAG-3 was increased in CD4+T cells of active pulmonary tuberculosis patients and decreased after anti-tuberculosis treatment.It might affect the ability of CD4+T cells to secrete IFN-γ,to impair the ability of CD4+T cells against Mycobacterium tuberculosis.
NETL
NSTL
万方数据
