Expression of SOX9 and SOX11 in ovarian cancer tissue and their relationship with prognosis
Objective To investigate the expression of sex-determining region Y box 9(SOX9)and sex-determining region Y box 11(SOX11)in ovarian cancer tissue and their relationship with prognosis.Methods From July 2018 to June 2020,143 ovarian cancer patients who underwent surgical treatment in the Beijing Daxing District People's Hospital were collected as the study subjects.Among them,136 patients underwent a 3-year follow-up and were separated into a survival group(n=85)and a death group(n=51)based on their 3-year survival status.Real-time fluorescence quantitative PCR(qRT-PCR)was applied to detect the expression levels of SOX9 and SOX11 in ovarian cancer tissues and adjacent tissues.Kaplan-Meier method was applied to plot survival curves;multi factor Cox regression model was applied to analyze the factors that affected the prognosis of ovarian cancer patients.Receiver operating characteristic(ROC)curve was applied to analyze the predictive value of SOX9 and SOX11 levels in ovarian cancer tissue for prognosis.Results The expression levels of SOX9 and SOX 11 in ovarian cancer tissue were higher than those in adjacent tissues;all the differences were statistically significant(t=29.530,27.908;all P＜0.05).The expression levels of SOX9 and SOX 11 in ovarian cancer tissues in the death group were higher than those in adjacent tissues;all the differences were statistically significant(t=8.080,9.341;all P＜0.05).There was a statistically obvious difference between the survival group and the death group in terms of differentiation,tumor diameter,lymph node metastasis,and Federation International of Gynecology and Obstetrics(FIGO)staging;all the differences were statistically significant(x2=6.170,4.077,9.353,12.455;all P＜0.05).The 3-year survival rate of the SOX9 high expression group(48.61％)was lower than that of the SOX9 low expression group(78.13％);the 3-year survival rate of the SOX 11 high expression group(51.43％)was lower than that of the SOX 11 low expression group(74.24％);all the differences were statistically significant(x2=14.202,9.032;all P＜0.05).SOX9,SOX11,degree of differentiation,lymph node metastasis,and FIGO staging were prognostic factors for ovarian cancer patients(HR=1.367,1.382,1.329,3.154,3.463;all P＜0.05).ROC results showed that the area under the curve(AUC)of SOX9 expression level in predicting the prognosis of ovarian cancer patients was 0.803,with a sensitivity of 66.67％and a specificity of 85.88％;the AUC of SOX 11 expression level in predicting the prognosis of ovarian cancer patients was 0.784,with a sensitivity of 68.63％and a specificity of 87.06％.The AUC of combination of SOX9 and SOX11 in predicting the prognosis of ovarian cancer patients(0.869)was obviously higher than the AUC predicted solely by SOX9 expression level and AUC predicted solely by SOX 11 expression level;all the differences were statistically significant(Z=2.099,2.294,P=0.036,0.022).Conclusion SOX9 and SOX 11 were highly expressed in ovarian cancer tissue,and their combination had high predictive value for the prognosis of ovarian cancer patients.
Ovarian cancerSex-determining region Y box 9Sex-determining region Y box 11Prognosis
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