Detection and clinical significance of peripheral blood double-negative T cells in patients with focal epilepsy
Objective To investigate the expression level of peripheral blood double-negative T cells(DNT,CD4-CD8-)in patients with focal epilepsy and its clinical significance.Methods A total of 89 patients with focal epilepsy were collected from the Department of Neurology,the Affiliated Hospital of Xuzhou Medical University from 2021 to 2023.Forty-two healthy individuals during the same period were selected as the control group.Peripheral blood samples were collected from all study subjects to detect the levels of T lymphocyte subsets and cytokines[interleukin-1 β(IL-1 β),IL-2,IL-4,IL-5,IL-6,IL-8,IL-10,IL-12p70,IL-17,interferon-α(IFN-α),IFN-γ,tumor necrosis factor-α(TNF-α)]in peripheral blood.Correlation analysis between DNT cells,cytokines,and T cell subsets was conducted,and the predictive value was evaluated by receiver operating characteristic(ROC)curve.Results The levels of DNT cells,IL-iβ,IL-5,IL-6,IFN-γ,and TNF-α in patients with focal epilepsy were significantly higher than those in the control group,the differences were statistically significant(Z=-6.15,-3.20,-3.09,-3.20,-2.41,-2.44;all P＜0.05).However,there was no significant difference in the levels of IL-2,IL-4,IL-8,IL-10,IL-12p70,IL-17,IFN-α,CD4+T,and CD8+T cells between the two groups(all P＞0.05).DNT cells showed a positive correlation with IL-17 and TNF-α(r=0.221,0.258;all P＜0.05)while exhibiting a negative correlation with CD4+T cells(r=-0.332,P＜0.05),but no significant correlation was found with other indicators(P＞0.05).ROC curve analysis showed that the area under the curve of DNT,TNF-α,IL-1β,IL-5,IL-6,IFN-γlevels and combined detection for diagnosing focal epilepsy were 0.816,0.785,0.674,0.668,0.674,0.631 and 0.858,respectively.The corresponding sensitivities were 64.04％,77.53％,64.04％,69.66％,48.31％,68.54％and 67.42％,respectively,and the corresponding specificities were 92.31％,69.05％,71.43％,57.14％,83.33％,57.14％and 95.24％,respectively.Conclusion The level of DNT cells in peripheral blood was significantly increased in patients with focal epilepsy and was correlated with IL-17,TNF-α,and CD4+T cells,suggesting their potential role in the pathogenesis of focal epilepsy.
Focal epilepsyDouble-negative T cellsCytokineNeuroinflammation
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