Feasibility study and analysis of establishing a mouse model of hepatic alveolar echinococcosis by hepatic portal vein infection
Objective Discusses the feasibility of establishing a mouse model of hepatic alveolar echinococcosis through the hepatic portal vein,provide a good modeling method and basis for clinical experimental research.Methods In this experiment,twenty C57 mice were used to establish a model of hepatic alveolar echinococcosis.First,the long-clawed gerbils whose abdomens have been infected with alveolar Echinococcus were aseptically killed.The abdominal cavity of the gerbil was cut open,the cystic echinococcal tissue was removed,and a suspension with a concentration of 20%was prepared(20 000 protoscolex cells/mL).The 200-300 μL suspension was injected into C57 mice via the hepatic portal vein.During the experiment,2 mice died of surgery and 2 of causes of anesthesia,with a mortality rate of 20%.Of the remaining 16 mice,4 were not successfully infected,uesd as non-infected group,12 mice successfully infected were used as the infection group,the infection modeling success rate was 75%.The mice were fed normally for 10 weeks after the operation.Finally,abdominal ultrasound imaging,nuclear magnetic resonance imaging,and histopathology were used to observe the liver lesions of the mice.Results The results showed that there were no abnormalities in the size,morphology,and signal of the liver in the non-infected group mice.The infection group ultrasound showed clear lesions in the left lobe of the liver,with clear boundaries and uneven echoes,mixed high and low echoes,and blood flow visible inside.Magnetic resonance imaging showed clear lesions in the left outer lobe of the liver in the infection group,with clear boundaries.The lesions were mainly characterized by slightly longer T2 signals,and multiple small cystic shadows were visible.The pathological results of the non-infected group were normal liver.Cystic vesicles and fibrous tissue can be observed in the liver lesions of the infection group,and there are many fibrous components around the vesicles.Inflammatory reaction zones can be seen around the periphery,containing inflammatory cells,microvessels,and fibrous tissue.Conclusions The method of trans-hepatic portal vein infection could establish a model of hepatic alveolar echinococcosis.This method was simple and feasible,and had a relatively high successful rate.
Hepatic alveolar echinococcosisHepatic portal veinNuclear magnetic resonance imaging
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