Expression and significance of serum LCN2,lncRNA-OIS1 and miR-3607-3p in patients with cervical cancer complicated with HPV infection
Objective To investigate the expression and significance of serum lipid carrier protein 2(LCN2),long non-coding RNA-OIS1(lncRNA-OIS1)and micrornA-3607-3p(miR-3607-3p)in patients with cervical cancer complicated with human papillomavirus(HPV)infection.Methods A total of 80 patients with cervical cancer admitted to the Third Hospital of Wuhan from May 2017 to May 2022 were selected as observation group,and 70 patients with cervical intraepithelial neoplasia were selected as control group.The levels of serum LCN2,lncRNA-OIS1,miR-3607-3p and HPV viral load and HPV positive expression rate were compared between the two groups.Pearson correlation analysis was used to analyze the correlation between serum LCN2,lncRNA-OIS1,miR-3607-3p and HPV viral load,HPV positive expression rate in patients with cervical cancer;multiple logistic regression was used to analyze the risk factors affecting the incidence of cervical cancer combined with HPV infection.Results The serum LCN2 level,HPV viral load and HPV positive expression rate in the observation group were(84.01±18.47)pg/mL,(1 171.40±232.33)RLU/CO and 100.00％,respectively,which were significantly higher than those in the control group[(43.25±12.83)pg/mL,(669.50±100.32)RLU/CO,45.71％];the levels of lncRNA-OIS1 and miR-3607-3p were(0.70±0.15)and(0.33±0.10),respectively,which were significantly lower than those in the control group[(0.83±0.17,(0.65±0.14)];the differences were statistically significant(t/x2=15.480,16.753,58.163,4.976,16.251;all P＜0.05).Pearson correlation analysis showed that serum LCN2 level was positively correlated with HPV viral load and HPV positive expression rate(r=0.515,0.430,both P＜0.05),while lncRNA-OIS1 and miR-3607-3p levels were negatively correlated with HPV viral load and HPV positive expression rate(r=-0.600,-0.502;-0.581,-0.459;all P＜0.05.Logistic regression analysis showed that serum LCN2 was a risk factor for cervical cancer complicated with HPV infection,and serum lncRNA-OIS1 and miR-3607-3p were protective factors for cervical cancer complicated with HPV infection;the differences were statistically significant(all P＜0.05).Conclusion In patients with cervical cancer complicated with HPV infection,serum LCN2 had significantly high expression level,while lncRNA-OIS1 and miR-3607-3p had significantly low expression levels,which were closely related to HPV viral load and HPV positive expression rate.
Cervical cancerHuman papillomavirusLipid carrier protein 2Long non-coding RNA-OIS1Micrornas-3607-3p
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