Distribution of pathogens and levels of serum HMGB1,IL-8 and CRP in patients with diabetic foot infection
Objective To explore the distribution of pathogens and levels of serum high mobility group protein Bl(HMGB1),interleukin-8(IL-8)and C-reactive protein(CRP)in patients with diabetic foot infection.Methods A total of 104 patients with diabetic foot infection of Liangzhou Hospital in Wuwei City from January 2023 to December 2023 were selected as the infected group,and another 60 patients with uninfected diabetic foot in the same period were selected as the non-infected group,and according to the distribution of pathogens,the patients in the infected group were divided into gram-negative bacteria(n=37),gram-positive bacteria(n=30),and mixed infection(n=37).According to clinical grading standard of diabetic foot infection in Infectious Diseases Society of America,patients with infection were divided into mild infection group(n=40),moderate infection group(n=33)and severe infection group(n=31).The levels of HMGB1,IL-8 and CRP in patients with different pathogens infection and infection degree were compared.The correlations between the severity of diabetic foot infection and HMGB1,IL-8,CRP levels were analyzed by Spearman.Results In the 104 patients with diabetic foot infection,there were 148 strains of pathogens,including 61 strains of gram-positive bacteria(41.22％),83 strains of gram-negative bacteria(56.08％)and 4 strains of fungi(2.70％).The levels of HMGB1,IL-8 and CRP in infection group were significantly higher than those in non-infection group,the differences were statistically significant(t=8.766,13.785,8.419;all P＜0.05).The levels of HMGB1,IL-8 and CRP in the mixed infection group were higher than those of gram-negative and gram-positive bacteria,the differences were statistically significant(F=37.464,59.860,37.000;all P＜0.05).The levels of HMGB1,IL-8 and CRP in the severe infection group were higher than those in the moderate infection group and mild infection group,the differences were statistically significant(F=44.821,64.281,58.025;all P＜0.05).The results of Spearman analysis showed that the severity of diabetic foot infection was positively correlated with levels of HMGB1,IL-8 and CRP(r=0.678,0.732,0.726;all P＜0.05).Conclusions Gram-negative bacteria were common in patients with diabetic foot infection.The levels of serum HMGB1,IL-8 and CRP were significantly increased after diabetic foot infection,and the increase in mixed infection group was greater than that in gram-negative bacteria group and gram-positive bacteria group.The levels of serum HMGB1,IL-8 and CRP were all correlated with the severity of infection.
Diabetic foot infectionPathogen distributionHigh mobility group protein B1Interleukin-8C-reactive protein
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