Pathogenic characteristics of Vibrio parahaemolyticus in Guangzhou from 2020 to 2022
Objective To analyze the serotype,virulence genes and gene characteristics of Vibrio parahaemolyticus isolates from Guangzhou,and provide a scientific basis for prevention and control of Vibrio parahaemolyticus.Methods The serotypes,virulence genes and pulse-field gel electrophoresis(PFGE)type of all the isolated stains of Vibrio parahaemolyticus were detected during 2020-2022.Homology analysis was performed using the comparison function of the Chinese Pathogen Identification Net.Results O10∶K4(50.8％,67/132)and O3∶K6(34.8％,46/132)were the predominant serotypes of the 132 Vibrio parahaemolyticus strains isolated.Virulence gene detection indicated all strains were detected positive for tlh genes(100.0％,132/132),130 isolates expressed tdh genes(98.5％,130/132),only 2 isolates expressed trh genes(1.5％,2/132).These strains could be divided into 31 PFGE patterns,and the similarity coefficients were between 23.54％-100.00％.It belonged to 6 clone clusters.Conclusions O10∶K4 and O3∶K6 were the most dominant serotypes in Vibrio parahaemolyticus strains from foodborne disease in Guangzhou,and tdh was detected in most of the strains.PFGE showed there was great diversity in this gene.
Vibrio parahaemolyticusSerotypesVirulence genePulse-field gel electrophoresis
NETL
NSTL
万方数据
