Research on serum biomarkers of heat acclimation based on metabolomics
Objective To explore the characteristics of metabolic product changes in heat acclimation(HA)enhancing the body's heat tolerance,and screen specific biomarkers for heat acclimation.Methods Fourteen C57BL/6 mice were randomly divided into a control(Con)group and a heat acclimation(HA)group.The HA group mice were established daily in an environmental simulation chamber with a temperature of(35±1)℃ and a relative humidity of(60±5)％for 4 consecutive weeks.The next day after the end,the mice were anesthetized and orbital blood was collected,and serum was separated.Liquid chromatograph mass spectrometer technology was used to detect changes in the main metabolites in the serum of HA group mice.Results Principal component analysis(PCA)and orthogonal partial least squares discriminant analysis(OPLS-DA)showed significant differences between the Con group and the HA group samples.Compared with the Con group,a total of 51 metabolites with significant differences were identified in the serum of HA group mice,of which 24 were upregulated and 27 were downregulated(variable importance in projection＞1,P＜0.05).These differential metabolites were mainly concentrated in pathways such as amino acid metabolism,carbohydrate metabolism,cofactor and vitamin metabolism,and digestive system.The Kyoto encyclopedia of genes and genomes(KEGG)pathway enrichment analysis showed these genes were mainly involved in protein digestion and absorption(P＜0.001),hypoxia inducible factor-1(HIF-1)signaling pathway(P=0.001),central carbon metabolism of cancer(P=0.001),digestion and absorption of vitamins(P=0.002),glucagon signaling pathway(P=0.005),D-glutamine and D-glutamic acid metabolism(P=0.013)and oxidative phosphorylation(P=0.019).Based on these metabolic pathways,a set of biomarkers closely related to heat acclimation were determined.Conclusion There were significant changes in serum metabolites in heat acclimatized mice,and these metabolites might serve as biomarkers for assessing the body's heat tolerance.
万方数据
维普
