The structure of vaginal microbiota in RVVC patients and the antibacterial effect of charred Sanguisorbae Radix alcohol extract
Objective To investigate the characteristics of vaginal microbiota in patients with recurrent vulvovaginal candidiasis(RVVC)and the inhibitory effect of charred Sanguisorbae Radix alcohol extract on clinically isolated Candida albicans.Methods A total of 15 RVVC patients(RVVC group)and 10 healthy women(control group)attending Nanjing Drum Tower Hospital from September to December 2022 were enrolled as the research subjects.The distribution characteristics of vaginal microbiota in RVVC patients were analyzed by 16S rRNA and internal transcribed spacer high throughput sequencing technology and the functional prediction of fungi was based on FUNGuild database.In addition,Candida albicans in clinical samples were isolated and cultured to observe the inhibitory effect of charred Sanguisorbae Radix alcohol extract was determined by plate colony count method.Results The vaginal bacteria of RVVC patients were dominated by Lactobacillus genus,with a predominance of Lactobacillus inert species.Compared with the control group,theα diversity of vaginal fungal microbiota in RVVC patients was significantly reduced(P＜0.05).NMDS showed clear classification of two groups and LEfSe analysis found that the relative abundance of Candida and Paramycosphaerella was increased and Candida albicans was the dominant fungus.FUNGuild functional classification predicted that undefined Saprotrophh was the most abundant group in the RVVC group,higher than its abundance in control group.In addition,compared to the blank control group,the number of Candida albicans colonies decreased,the differences were statistically significant(t=8.350,8.722,9.137,9.144;all P＜0.05),while the number of colonies of Lactobacillus curlicus increased with the increase of the concentration of charred Sanguisorbae Radix alcohol extract(25,50,100,200 µg/mL),the differences were statistically significant(t=9.952,6.288,10.440,8.742;all P＜0.05).Conclusions The vaginal microbiota of RVVC patients was disordered and mainly dominated by Lactobacillus iners and Candida albicans.The alcohol extract of charred Sanguisorbae Radix had an inhibitory effect on clinically isolated Candida albicans and promoted the growth of Lactobacillus crispatus.This study could provide new ideas and theoretical basis for the research and development of traditional Chinese medicine for the prevention and treatment of RVVC.
万方数据
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