Typing and antimicrobial resistance testing of Salmonella isolates from diarrhea patients in Zunyi
Objective Investigate the typing and drug resistance characteristics of Salmonella isolates in diarrhea patients in Zunyi city.Methods Serotyping of Salmonella strains isolated from diarrhea patients in Zunyi from 2020 to 2022 was performed by serum agglutination method,and molecular typing was performed by pulsed-field gel electrophoresis(PFGE);drug resistance was determined by broth dilution method.BioNumerics software was used for result analysis.Results 59 strains of human Salmonella were identified into 8 serotypes,with Salmonella typhimurium and Salmonella enteritidis accounting for 52.54％and 33.90％,respectively;PFGE banding showed polymorphism,with 13 100％homologous strains in 6 families and 19 highly homologous strains in 8 families;drug sensitivity results showed that 93.22％of the strains were resistant to 17 antibiotics,and the multidrug resistance rate reached 76.27％.Among them,the resistance to ampicillin,streptomycin and tetracycline was high,and it was relatively sensitive to amikacin,tigecycline,ertapenem,meropenem,ceftazidime/avibactam and azithromycin;Salmonella typhimurium was highly resistant to streptomycin,ampicillin and tetracycline,and Salmonella enteritidis was highly resistant to ampicillin and nalidixic acid.Conclusions The dominant serotypes of human Salmonella in Zunyi city were Salmonella typhimurium and Salmonella enteritidis,which were genetically diverse,had a high resistance rate to antibiotics,and had serious multidrug resistance.There were differences in the resistance rates of different serotypes,and the monitoring of multidrug-resistant strains and the supervision of the use of antimicrobial drugs should be strengthened.
DiarrheaSalmonellaSerotypePulsed-field gel electrophoresisDrug resistance
万方数据
维普
