Distribution characteristics of Aedes albopictus symbiotic Elizabethkingia and its effects on egg hatching and larval growth
Objective To analyze the distribution of Elizabethkingia in different development stages,tissues and field populations of Aedes albopictus,and study its effects on the hatching of eggs and larval growth of Aedes albopictus.Methods The bacteria were obtained from the laboratory raised Aedes albopictus.The genus was comprehensively identified by scanning electron microscopy,physicochemical reactions and phylochemical tree analysis.The distribution of Elizabethkingia in different developmental stages,tissues and field populations of Aedes albopictus was detected by absolute quantitative method.The bacteria were divided into control group,antibiotic group,AdeAl-3 group and antibiotic+AdeAl-3 group,and the changes of egg hatching rate,larval survival rate and pupation rate in different groups were observed.Results A strain of bacterium AdeAl-3 was isolated from Aedes albopictus at different developmental stages,and identified as Elizabethkingia miricola.AdeAl-3 was abundant in pupae and larvae of Aedes albopictus,less abundant in adult mosquitoes(F=18.11,P＜0.05).AdeAl-3 was mainly distributed in the midguts of Aedes albopictus,and salivary glands and ovaries were also detected but the content was less(F=588.70,P＜0.05).AdeAl-3 was present in the Aedes albopictus field population.Compared with the control group,AdeAl-3 group had no significant effects on the hatching rate of mosquito eggs and survival ratio of larvae(x2=0.06,0.01;all P＞0.05).There were significant differences in larval pupation rates in the control group,AdeAl-3 group and antibiotic+AdeAl-3 group.The pupation rate of larvae in AdeAl-3 group was increased compared with that of the control group,the difference was statistically significant(x2=5.62,P＜0.05).Conclusion The AdeAl-3 was a bacterium that was ubiquitous in Ae.albopictus population,and had no significant effects on the hatching and larvae survival,but could increase the pupation rate of larvae,suggesting it might be suitable for engineering bacterial modification.
Aedes albopictusElizabethkingia miricolaHatching of eggsLarval growth
万方数据
维普
