Drug resistance characteristics and adhesin gene expression of Escherichia coli in children with urinary tract infection
Objective To investigate the drug resistance characteristics and adhesion gene expression of Escherichia coli in children with urinary tract infection.Methods A total of 160 non-repeated Escherichia coli strains isolated from urine samples in Baoding Hospital,Beijing Children's Hospital,Capital Medical University from October 1,2019 to October 1,2022 were selected for drug sensitivity test.According to the results of drug sensitivity test,they were divided into sensitive group(n=80)and resistant group(n=80).The fimH,fimA,fimB,papA,papB,papC and papGⅡ genes were detected by PCR.The relative expression levels were detected by real-time fluorescence quantitative PCR.The adhesion ability of type Ⅰfimbriae and type P fimbriae was detected by yeast cell adhesion test and red blood cell agglutination test.Results The resistance rate of the sensitive group to ampicillin,sulfamethoxazole/trimethoprim and levofloxacin was＞10％,the resistance rate to ciprofloxacin,cefazolin,tobramycin and amtronam was＜10％,and the resistance rate to other biochemicals was completely sensitive.The resistance rate of resistant group to cefoperazone/sullbactam and imipenem was ≤4％,and the resistance rate to other biochemicals was＞50％.The detection rate of papGⅡ gene in the resistant group(40.00％)was significantly higher than that in the sensitive group(15.00％)(x2=12.541,P＜0.05);There was no significant difference in the detection rate of papA,papB and papC genes between two groups(all P＞0.05).The expression levels of fimH,fimB,papB,papC and papGⅡ genes in the sensitive group were higher than those in the resistant group(t=20.575,33.727,3.095,19.461,4.275;all P＜0.05);There was no significant difference in the expression levels of fimA and papA genes between the two groups(both P＞0.05).The adhesion rate of type Ⅰ pili in the sensitive group was higher than that in the resistant group(x2=13.231,P＜0.05).Conclusions The decrease of adhesion ability of type Ⅰ fimbriae in the resistant group was related to the adaptive cost of the strain.The transcription and deletion of other genes encoded by fim and pap gene clusters might affect the adhesion function of type Ⅰ fimbriae and type P fimbriae.
万方数据
维普
