The expression and clinical significance of sPD-L1 and sFas in serum of HIV/HBV infected patients
Objective To study the expression and clinical significance of soluble programmed death factor ligand-1(sPD-L1)and soluble apoptosis related factor(sFas)in the serum of patients with human immunodeficiency virus/hepatitis B virus(HIV/HBV)coinfection.Methods A total of 138 HBV infected patients who were diagnosed and treated in the Guizhou Aerospace Hospital from February 2018 to February 2021 were selected as the research subjects.They were divided into the HIV/HBV group(HIV/HBV coinfection,n=60)and the HBV group(simple HBV infection,n=78).A total of 50 healthy individuals who underwent physical examinations in our hospital during the same period were used as the control group.Enzyme linked immunosorbent assay was used to detect serum sPD-L1 and sFas levels in each group.Pearson correlation analysis was used to analyze the correlation between serum sPD-L1,sFas levels and clinical indicators.Multivariate logistic regression analysis was conducted to investigate the factors influencing the co infection of HIV/HBV.The diagnostic value of sPD-L1,sFas and combined detection in the diagnosis of HIV/HBV coinfection by analyzing the receiver operating characteristic curve of subjects.Results The levels of serum alanine transaminase,aspartate transaminase,sPD-L1,sFas,tumor necrosis factor-α(TNF-α)and interleukin-6(IL-6)in HIV/HBV group were significantly higher than those in HBV group and control group(all P＜0.05);the levels of serum alanine transaminase,aspartate transaminase,sPD-L1,sFas,TNF-α and IL-6 in HBV group were significantly higher than those in control group(all P＜0.05).There were significant differences in the above indexes among the three groups(F=682.191,1 149.180,166.771,437.213,382.011,754.180;all P＜0.05).There was a significant positive correlation between serum sPD-L1,sFas levels and HIV RNA,HBV DNA,alanine transaminase,aspartate transaminase,TNF-α,IL-6 in patients with HIV/HBV coinfection(r=0.640,0.701,0.534,0.551,0.603,0.615;0.617,0.653,0.498,0.434,0.701,0.723;all P＜0.05),while a significant negative correlation with peripheral blood CD4+T lymphocyte count(r=-0.662,-0.669;both P＜0.05).sPD-L1,sFas were independent risk factors for HIV/HBV coinfection(both P＜0.05).The area under the curve of serum sPD-L1 and sFas combined detection for the diagnosis of HIV/HBV coinfection was 0.893,which was significantly higher than that of serum sPD-L1 and sFas alone(0.820,0.721)(Z=2.302,4.918,both P＜0.05).Conclusions The serum levels of sPD-L1 and sFas in patients with HIV/HBV coinfection were elevated,which were independent risk factors for HIV/HBV coinfection.The combined detection of serum sPD-L1 and sFas had high diagnostic value for HIV/HBV coinfection.
Human immunodeficiency virusHepatitis B virusSoluble programmed death factor ligand-1Soluble apoptosis related factor
万方数据
